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cell suspensions  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cell suspensions
    Cell Suspensions, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 3305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fcr+blocking/FcR+Blocking+Reagent%2C+human/pm42521848-287-2-23
    Average 97 stars, based on 3305 article reviews
    cell suspensions - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: An improved method for high-purity isolation of oligodendrocytes from neonatal and adult mouse brain
    Article Snippet: Cell numbers were determined using a Countess 3 FL Automated Cell Counter (Invitrogen). .. For neonatal PDGFRα + OPC isolation, cell pellet from one brain was resuspended in 78 μL DPBS/BSA buffer, incubated with 10 μL FcR blocking reagent, and then with 12 μL anti-PDGFRα microBeads (Miltenyi Biotec, 130-101-502). ..

    Incubation:

    Article Title: An improved method for high-purity isolation of oligodendrocytes from neonatal and adult mouse brain
    Article Snippet: Cell numbers were determined using a Countess 3 FL Automated Cell Counter (Invitrogen). .. For neonatal PDGFRα + OPC isolation, cell pellet from one brain was resuspended in 78 μL DPBS/BSA buffer, incubated with 10 μL FcR blocking reagent, and then with 12 μL anti-PDGFRα microBeads (Miltenyi Biotec, 130-101-502). ..

    Blocking Assay:

    Article Title: An improved method for high-purity isolation of oligodendrocytes from neonatal and adult mouse brain
    Article Snippet: Cell numbers were determined using a Countess 3 FL Automated Cell Counter (Invitrogen). .. For neonatal PDGFRα + OPC isolation, cell pellet from one brain was resuspended in 78 μL DPBS/BSA buffer, incubated with 10 μL FcR blocking reagent, and then with 12 μL anti-PDGFRα microBeads (Miltenyi Biotec, 130-101-502). ..

    Article Title: A STAT1/ETC/GBP1 axis represents a potential therapeutic target for noncommunicable granulomatous skin disease
    Article Snippet: .. Unspecific binding was blocked with FcR blocking (Miltenyi Biotec, 130-059-901), and dead cells were excluded by staining them with Fixable Viability Dye eFluor 780 (Invitrogen, 65-0865-14). .. Extracellular staining (CD86 and CD40) was performed in phosphate-buffered saline (PBS) with 2 % FBS (Pan-Biotech, P30-3302) and 0.1% sodium azide [fluorescence-activated cell sorting (FACS) buffer] for 20 min at 4°C.

    Article Title: A chemogenetic CRISPR knockout screen identifies a ubiquitin-mediated calcineurin-BMP pathway relay in T cell activation
    Article Snippet: .. T cells were washed with PBS and resuspended in 100 μL of FACS Buffer and 10 μL of FcR blocking reagent, Miltenyi Biotec (Cat. 130-059-901). ..

    Article Title: The nucleolar protein NOP16 suppresses TGF-β-regulated IgA class switch recombination in B cells by regulating AID induction.
    Article Snippet: .. Flow cytometry and cell sorting Cells were washed with cold FACS buffer, blocked with FcR Blocking Reagent, mouse (Miltenyi Biotec), and stained with PE– anti-IgA monoclonal antibody (Southern Biotech, Birmingham, Alabama, USA; 1:200). .. All flow cytometry samples were analyzed using a FACSVerse instrument (Becton, Dickinson and Company, Franklin Lakes, New Jersey, USA), and data were processed using FlowJo software (Becton, Dickinson and Company).

    Article Title: The in vitro acquisition of CD11a by gastric cancer cells after contact with T cells via trogocytosis increases adhesion to endothelial cells.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Sex-Dimorphic Neural Memory Shapes Pancreatic Tissue Resilience
    Article Snippet: Slide were allowed to cool down under running tap water until reaching room temperature and blocked for 45 minutes with blocking solution composed of 1% BSA (Sigma), 10% FBS (Fisher Scientific), 0.2% Triton X-100 (Sigma). .. For stains using mouse monoclonal antbodies, blocking buffer additionally contained FcR Blocking reagent 1:100 (Miltenyi). ..

    Article Title: Protocol for isolating viable human central nervous system T cells
    Article Snippet: LIVE/DEAD Fixable Viability Dye eFluor 780 (1:1,000 dilution) , Thermo Fisher Scientific , Cat# 65-0865. .. FcR Blocking Reagent , Miltenyi Biotec , Cat# 130-059-901 RRID: AB_2892112. ..

    Article Title: A glial source of noradrenaline shapes synaptic integration and motor adaptation
    Article Snippet: .. Fc receptors were blocked with the accompanying FcR Blocking Reagent, then cells were labeled with Anti-ACSA-2 MicroBeads (130-097-678, Miltenyi Biotech, France) for 15 minutes at 4 °C and loaded onto magnetic selection (MS) columns for positive selection. ..

    Binding Assay:

    Article Title: A STAT1/ETC/GBP1 axis represents a potential therapeutic target for noncommunicable granulomatous skin disease
    Article Snippet: .. Unspecific binding was blocked with FcR blocking (Miltenyi Biotec, 130-059-901), and dead cells were excluded by staining them with Fixable Viability Dye eFluor 780 (Invitrogen, 65-0865-14). .. Extracellular staining (CD86 and CD40) was performed in phosphate-buffered saline (PBS) with 2 % FBS (Pan-Biotech, P30-3302) and 0.1% sodium azide [fluorescence-activated cell sorting (FACS) buffer] for 20 min at 4°C.

    Staining:

    Article Title: A STAT1/ETC/GBP1 axis represents a potential therapeutic target for noncommunicable granulomatous skin disease
    Article Snippet: .. Unspecific binding was blocked with FcR blocking (Miltenyi Biotec, 130-059-901), and dead cells were excluded by staining them with Fixable Viability Dye eFluor 780 (Invitrogen, 65-0865-14). .. Extracellular staining (CD86 and CD40) was performed in phosphate-buffered saline (PBS) with 2 % FBS (Pan-Biotech, P30-3302) and 0.1% sodium azide [fluorescence-activated cell sorting (FACS) buffer] for 20 min at 4°C.

    Article Title: The nucleolar protein NOP16 suppresses TGF-β-regulated IgA class switch recombination in B cells by regulating AID induction.
    Article Snippet: .. Flow cytometry and cell sorting Cells were washed with cold FACS buffer, blocked with FcR Blocking Reagent, mouse (Miltenyi Biotec), and stained with PE– anti-IgA monoclonal antibody (Southern Biotech, Birmingham, Alabama, USA; 1:200). .. All flow cytometry samples were analyzed using a FACSVerse instrument (Becton, Dickinson and Company, Franklin Lakes, New Jersey, USA), and data were processed using FlowJo software (Becton, Dickinson and Company).

    FACS:

    Article Title: A chemogenetic CRISPR knockout screen identifies a ubiquitin-mediated calcineurin-BMP pathway relay in T cell activation
    Article Snippet: .. T cells were washed with PBS and resuspended in 100 μL of FACS Buffer and 10 μL of FcR blocking reagent, Miltenyi Biotec (Cat. 130-059-901). ..

    Article Title: The nucleolar protein NOP16 suppresses TGF-β-regulated IgA class switch recombination in B cells by regulating AID induction.
    Article Snippet: .. Flow cytometry and cell sorting Cells were washed with cold FACS buffer, blocked with FcR Blocking Reagent, mouse (Miltenyi Biotec), and stained with PE– anti-IgA monoclonal antibody (Southern Biotech, Birmingham, Alabama, USA; 1:200). .. All flow cytometry samples were analyzed using a FACSVerse instrument (Becton, Dickinson and Company, Franklin Lakes, New Jersey, USA), and data were processed using FlowJo software (Becton, Dickinson and Company).

    Flow Cytometry:

    Article Title: The nucleolar protein NOP16 suppresses TGF-β-regulated IgA class switch recombination in B cells by regulating AID induction.
    Article Snippet: .. Flow cytometry and cell sorting Cells were washed with cold FACS buffer, blocked with FcR Blocking Reagent, mouse (Miltenyi Biotec), and stained with PE– anti-IgA monoclonal antibody (Southern Biotech, Birmingham, Alabama, USA; 1:200). .. All flow cytometry samples were analyzed using a FACSVerse instrument (Becton, Dickinson and Company, Franklin Lakes, New Jersey, USA), and data were processed using FlowJo software (Becton, Dickinson and Company).

    Article Title: The in vitro acquisition of CD11a by gastric cancer cells after contact with T cells via trogocytosis increases adhesion to endothelial cells.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Magnetic Cell Separation:

    Article Title: The in vitro acquisition of CD11a by gastric cancer cells after contact with T cells via trogocytosis increases adhesion to endothelial cells.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Negative Control:

    Article Title: The in vitro acquisition of CD11a by gastric cancer cells after contact with T cells via trogocytosis increases adhesion to endothelial cells.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Labeling:

    Article Title: A glial source of noradrenaline shapes synaptic integration and motor adaptation
    Article Snippet: .. Fc receptors were blocked with the accompanying FcR Blocking Reagent, then cells were labeled with Anti-ACSA-2 MicroBeads (130-097-678, Miltenyi Biotech, France) for 15 minutes at 4 °C and loaded onto magnetic selection (MS) columns for positive selection. ..

    Selection:

    Article Title: A glial source of noradrenaline shapes synaptic integration and motor adaptation
    Article Snippet: .. Fc receptors were blocked with the accompanying FcR Blocking Reagent, then cells were labeled with Anti-ACSA-2 MicroBeads (130-097-678, Miltenyi Biotech, France) for 15 minutes at 4 °C and loaded onto magnetic selection (MS) columns for positive selection. ..



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